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Lab-on-a-Chip and BioMEMS Devices
Lab-on-a-chip devices (also known as micro-total-analytical systems or microTAS) are devices designed to miniaturize analytical or bioanalytical techniques and integrate them into a microfabricated format. Techniques such as chemical separations (electrophoresis, chromatography, etc) or immunoassays are incorporated into microfabricated systems (typically glass, silicon or polymers) with a goal of increasing performance, minimizing reagent requirements, and decreasing cost. BioMEMS devices are similar, typically focusing on MEMS (microelectromechanical systems) with biological applications.

We use lab-on-a-chip devices to capture, sort, culture, or study cells, study interfacial phenomena, and produce pharmaceuticals.

Selected Publications and Presentations on Lab-on-a-Chip and BioMEMS devices

Hawkins BG, Kirby BJ
"Electrothermal flow effects in insulating (electrodeless) dielectrophoresis systems," submitted, 2010.

Pratt ED, Huang C, Hawkins BG, Gleghorn JP, Kirby BJ
"Rare cell capture in microfluidic devices, submitted, 2010.

HTML version of Kirby: Micro- and Nanoscale Fluid Mechanics: Transport in Microfluidic Devices

Kirby BJ
"Micro- and Nanoscale Fluid Mechanics: Transport in Microfluidic Devices," Cambridge University Press, 2010.
click here for html version| Cambridge University Press

PDF version of Gleghorn, Pratt, Denning, Liu, Bander, Tagawa, Nanus, Giannakakou, Kirby:
Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunocapture (GEDI) and a prostate-specific antibody

Gleghorn JP, Pratt ED, Denning D, Liu H, Bander NH, Tagawa S, Nanus DM, Giannakakou PA, Kirby BJ
"Capture of circulating tumor cells from whole blood of prostate cancer patients using geometrically enhanced differential immunocapture and a prostate-specific antibody", Lab on a Chip, 10:27-29, 2010. doi pdf

Barbati AC, Fang C, Banker GA, Kirby BJ
"Microfluidic culture devices for observation of axonal organelle transport", ", MicroTAS 2009, 1-5 Nov 2009, Jeju, Korea.

Kondapalli SK, Connelly JT, Baeumner AJ, Kirby BJ
"On-chip electrophoretic concentration of liposomes for antibody-based viral biosensors", ", MicroTAS 2009, 1-5 Nov 2009, Jeju, Korea.

PDF version of Brian Kirby Nanofluidics review

Kirby BJ
"Nanofluidics" (book review), Materials Today, 12(5) 51, 2009. doi pdf

PDF version of Tandon Kirby: Transient zeta potential measuremnts in hydrophobic topas microfluidics substrates

Tandon VT, Bhagavatula S, Kirby BJ
"Transient Zeta Potential Measurements in Hydrophobic, TOPAS Microfluidic Substrates," Electrophoresis 30(15) 2656-2667, 2009. doi pdf

PDF version of Refolding of beta-galactosidase: microfluidic device for reagent metering and mixing and quantification of refolding yield 
Sowmya Kondapalli and Brian J. Kirby

Kondapalli S, Kirby BJ
"Refolding of beta-galactosidase: Microfluidic device for reagent metering and mixing and quantification of refolding yield," Microfluidics and Nanofluidics 7(2) 275-281, 2009. doi pdf

Hawkins BG, Gleghorn JP, Kirby BJ
"Dielectrophoresis for cell and particle manipulations," in Methods in Bioengineering: Biomicrofabrication and Biomicrofluidics, Ed. J.D. Zahn, Artech Press, 2009.

Kondapalli S, Kirby BJ
"Refolding of beta-galactosidase: Microfluidic device for reagent metering and mixing and quantification of refolding yield," AIChE 2008.

PDF version of George, Rana, Hawkins, Kirby: 
Microfluidic devices for terahertz spectroscopy of biomolecules

George PA, Hui W, Rana F, Hawkins BG, Smith AE, Kirby BJ
"Integrated microfluidic devices for terahertz spectroscopy of biomolecules", Optics Express, 16(3) 1577-1582 (2008). pdf text

Hawkins BG, Smith AE, Kirby BJ
"High-throughput, continuous-flow, dielectrophoretic screening of Mycobacterium smegmatis in coherently patterned, polymeric microchannels", MicroTAS 2007, Paris, France, October 2007.

PDF version of Tandon V Bhagavatula SK Nelson WC Kirby BJ:
Zeta potential and electroosmotic mobility in microfluidic devices
fabricated from hydrophobic polymers: 1. The origins of charge

Tandon V, Bhagavatula SK, Nelson WC, Kirby BJ
"Zeta potential and electroosmotic mobility in microfluidic devices fabricated from hydrophobic polymers: 1. The origins of charge", Electrophoresis 29(5):1092-1101, 2008. doi pdf

PDF version of Tandon V Kirby BJ:
Zeta potential and electroosmotic mobility in microfluidic devices 
fabricated from hydrophobic polymers: 2. Slip and interfacial water structure

Tandon V, Kirby BJ
"Zeta potential and electroosmotic mobility in microfluidic devices fabricated from hydrophobic polymers: 2. Slip and interfacial water structure", Electrophoresis 29(5):1102-1114, 2008. doi pdf

Hawkins BG, Smith AE, Kirby BJ
Gordon Research Conference on Microfluidics, Waterville Valley, NH, 2007.

PDF version of Benjamin G. Hawkins, A. Ezekial Smith, 
Yusef A. Syed, Brian J. Kirby: 
Continuous-Flow Particle Separation 
by 3D Insulative Dielectrophoresis 
Using Coherently Shaped, dc-Biased, ac Electric Fields

Hawkins BG, Smith AE, Syed YA, Kirby BJ
"Continuous-flow particle separation by 3D insulative dielectrophoresis using coherently shaped, DC-biased, AC electric fields," Analytical Chemistry, 2007. doi pdf text

Hawkins BG, Smith AE, Syed YA, Kirby BJ
"Electrokinetic particle sorting in cyclic olefin polymer microsystems," AIChE 2006 San Francisco, CA, Nov 2006.

PDF version of Mela, van den Berg, Fintschenko, Cummings, Simmons, Kirby: The zeta potential of cyclo-olefin polymer microchannels and its effects on insulative (electrodeless) dielectrophoresis particle trapping devices

Mela P, van den Berg A, Fintschenko Y, Cummings EB, Simmons BA, Kirby BJ
"The zeta potential of cyclo-olefin polymer microchannels and its effects on insulative (electrodeless) dielectrophoresis particle trapping devices," Electrophoresis 26:1792-1799 (2005). doi pdf text

PDF version of Reichmuth, Shepodd, Kirby: Microchip HPLC of peptides and proteins

Reichmuth DS, Shepodd TJ, Kirby BJ
"Microchip HPLC of peptides and proteins," Analytical Chemistry 77:2997-3000 (2005). doi pdf text

PDF version of Kirby, Reichmuth, Renzi, Shepodd, Wiedenman: Microfluidic routing of aqueous and organic flows at high pressure: Fabrication and characterization of integrated polymer microvalve elements

Kirby BJ, Reichmuth DS, Renzi RF, Shepodd TJ, Wiedenman BJ
"Microfluidic routing of aqueous and organic flows at high pressure: Fabrication and characterization of integrated polymer microvalve elements," Lab on a Chip 5:184-190 (2005). doi pdf text

PDF version of Reichmuth, Chirica, Kirby: Analysis of peptides using an integrated microchip HPLC-MS/MS system

Reichmuth DS, Chirica GS, Kirby BJ
"Analysis of peptides using an integrated microchip HPLC-MS/MS system," in MicroTAS 2004, Kluwer Academic Publishers (2004). pdf

PDF version of Reichmuth, Shepodd, Kirby: On-chip high-pressure picoliter injector for pressure-driven flow through porous media

Reichmuth DS, Shepodd TJ, Kirby BJ
"On-chip high-pressure picoliter injector for pressure-driven flow through porous media," Analytical Chemistry 76:5063-5068 (2004). doi pdf text

PDF version of Song, Singh, Kirby: Electrophoretic concentration of proteins at laser-patterned porous membranes

Song S, Singh AK, Kirby BJ
"Electrophoretic Concentration of Proteins at Laser-Patterned Porous Membranes," Analytical Chemistry 76:4589-4592 (2004). doi pdf text

PDF version of Song, Singh, Shepodd, Kirby: Microchip dialysis of proteins using in situ photopatterned nanoporous polymer membranes

Song S, Singh AK, Shepodd TJ, Kirby BJ
"Microchip dialysis of proteins using in situ photopatterned nanoporous polymer membranes", Analytical Chemistry 76:2367-2373 (2004). doi pdf text

PDF version of Kirby, Hasselbrink: The zeta potential of microfluidic substrates. 1. Theory, experimental techniques, and effects on separations

Kirby BJ, Hasselbrink, Jr. EF
"The Zeta Potential of Microfluidic Substrates. 1. Theory, experimental techniques, and effects on separations," Electrophoresis, 25:187-202 (2004). doi pdf text

PDF version of Kirby, Hasselbrink: The zeta potential of microfluidic substrates. 2. Data for polymers

Kirby BJ, Hasselbrink, Jr. EF
"The Zeta Potential of Microfluidic Substrates. 2. Data for polymers," Electrophoresis, 25:203-213 (2004). doi pdf text

PDF versino of Fintschenko, Kirby, Hasselbrink, Singh, Shepodd: Monolithic materials: miniature and microchip technologies

Fintschenko Y, Kirby BJ, Hasselbrink, Jr. EF, Singh AK, Shepodd TJ
"Monolithic Materials: Miniature and Microchip Technologies," in Monolithic Materials: Preparation, Properties, and Applications Elsevier, Amsterdam (2003). pdf

PDF version of Kirby, Wheeler, Zare, Fruetel, Shepodd: Programmable modification of cell adhesion and zeta potential in silica microchips

Kirby BJ, Wheeler AR, Zare RN, Fruetel JA, Shepodd TJ
"Programmable Modification of Cell Adhesion and Zeta Potential in Silica Microchips,"Lab On a Chip 3:5-10 (2003). doi pdf text

PDF version of Kirby, Shepodd, Hasselbrink: Voltage-addressable on/off microvalves for high-pressure microchip separations

Kirby BJ, Shepodd TJ, Hasselbrink, Jr. EF
"Voltage-Addressable On/Off Microvalves for High-Pressure Microchip Separations," Journal of Chromatography A 979:147-154 (2002). doi pdf

PDF version of Wheeler, Morishima, Kirby, Leach, Zare: CATH.a neuron cell analysis on a chip with micellar electrokinetic chromatography

Wheeler AR, Morishima K, Kirby BJ, Leach A, Zare RN
"CATH.a Neuron Cell Analysis on a Chip With Micellar Electrokinetic Chromatography," MicroTAS 2001, Kluwer Academic Publishers, (2001). pdf

AFM characterization of a zeonor surface which can be used to assist study of electrokinetic properties of microchannel substrates.